Review





Similar Products

e1b gfp tol2  (Addgene inc)
93
Addgene inc e1b gfp tol2
E1b Gfp Tol2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e1b gfp tol2/product/Addgene inc
Average 93 stars, based on 1 article reviews
e1b gfp tol2 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
tol2 transgenic vector e1b egfp tol2  (Addgene inc)
93
Addgene inc tol2 transgenic vector e1b egfp tol2
A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the <t>Tol2</t> Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.
Tol2 Transgenic Vector E1b Egfp Tol2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tol2 transgenic vector e1b egfp tol2/product/Addgene inc
Average 93 stars, based on 1 article reviews
tol2 transgenic vector e1b egfp tol2 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
plasmid vector e1b gfp tol2  (Addgene inc)
93
Addgene inc plasmid vector e1b gfp tol2
A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the <t>Tol2</t> Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.
Plasmid Vector E1b Gfp Tol2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid vector e1b gfp tol2/product/Addgene inc
Average 93 stars, based on 1 article reviews
plasmid vector e1b gfp tol2 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
750 bp mmp14b enh sequence  (Addgene inc)
93
Addgene inc 750 bp mmp14b enh sequence
A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the <t>Tol2</t> Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.
750 Bp Mmp14b Enh Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/750 bp mmp14b enh sequence/product/Addgene inc
Average 93 stars, based on 1 article reviews
750 bp mmp14b enh sequence - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
tol2 transgenic vector e1b gfp tol2 58  (Addgene inc)
93
Addgene inc tol2 transgenic vector e1b gfp tol2 58
A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the <t>Tol2</t> Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.
Tol2 Transgenic Vector E1b Gfp Tol2 58, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tol2 transgenic vector e1b gfp tol2 58/product/Addgene inc
Average 93 stars, based on 1 article reviews
tol2 transgenic vector e1b gfp tol2 58 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
tol2 plasmid  (Addgene inc)
93
Addgene inc tol2 plasmid
A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the <t>Tol2</t> Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.
Tol2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tol2 plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
tol2 plasmid - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the Tol2 Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.

Journal: bioRxiv

Article Title: Heart tube morphogenesis is regulated by segment-specific gene regulatory networks controlled by MEF2C

doi: 10.1101/2024.11.01.621613

Figure Lengend Snippet: A) Schematic of selection process to identify candidate MEFC-dependent enhancers from integrated snRNA-seq and snATAC-seq data. B) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at example loci containing IFT-specific (left), V-specific (middle), and OFT-specific (right) candidate enhancers (yellow highlights). C) Genome browser tracks displaying snATAC-seq accessibility profiles, MEF2C ChIP-seq occupancy profiles , and H3K27ac ChIP-seq occupancy profiles at the Myh6 / Myh7 locus, which contains two candidate enhancers (yellow highlights, MVEB1 and MVEB2) with IFT-specific altered accessibility that overlap with regions found in the VISTA Enhancer Browser database . D) Images of E11.5 mouse embryos from the Vista Enhancer Browser database demonstrating positive enhancer activity of regions that overlap with candidates MVEB1 and MVEB2. E) Schematic of the Tol2 Transgenesis assay used to screen candidate enhancers in zebrafish. Elements of this panel were created in BioRender. B, B. (2024) https://BioRender.com/h83r503 . F) Representative ventral view images of zebrafish embryos at 72 hpf injected with candidate enhancers that demonstrated positive activity in the heart. Boxed area in the representative brightfield image (left) indicates the anatomical region of interest captured in the fluorescent images. G) Schematic representation of the observed onset of enhancer activity for candidate enhancers that demonstrated positive activity in the heart. H) Representative ventral view images of zebrafish embryos at 24 and 72 hpf injected with MVEB2:eGFP or MVEB6:eGFP reporter constructs. Scale bars = 100 μm.

Article Snippet: The resulting fragments were cloned into the Tol2 transgenic vector E1b-eGFP-Tol2 ( ) (RRID: Addgene_37845) using the In-Fusion Cloning system (Takara Bio).

Techniques: Selection, ChIP-sequencing, Activity Assay, Injection, Construct